How do I determine the minimum number of observations needed per category in categorical predictor variables?

When conducting a simple linear regression analysis with one categorical predictor variable and power projected at .80, G*Power suggests a minimum sample size of n = 55. Now, assume that the...

10 January 2021 6,317 6 View

Advice to Create a Stable Cell Line with a T-to-A Mutation?

Does anyone have a recommendation on a methodology to create a stable cell line with a T-to-A mutation in a protein coding gene? We should be able to use CRISPR to induce a double-stranded break...

07 October 2020 2,504 1 View

How do I deal with the insufficient memory error in CST MWS?

I'm trying to analyze ship RCS in CST MWS. The ship length is about 120 meter. When I run the simulation with 1 MHz, it works. However, when I run it with 100 MHz, it turns out the error about...

10 May 2020 9,512 2 View

Collecting immune complexes from ELISA plate and running on mass spectrometer?

I performed an ELISA to quantify circulating immune complexes from blood sera using the C1q capture based assay from Quidel. I am interested to learn what the immune complexes are composed off and...

28 May 2019 9,045 1 View

Why must an antibody be at a conc. of 1-10 mg/ml for reduction/conjugation reactions?

I am attempting to conjugate an antibody to a maleimide-drug conjugate. All the TCEP reduction protocols I find specify a protein concentration of 1-10 mg/ml in some sort of buffer (sodium borate,...

08 March 2018 4,898 1 View

Can I copy one of my publications and save it as a file to send to you? What about copyright laws?

31 July 2017 4,694 4 View

Why does my Kd change when I alter the concentration of the constant component of my binding system?

I am measuring the binding of thrombin binding aptamer (TBA) to thrombin using fluorescence anisotropy. TBA is labelled with a fluorophore (5'-FAM) and was initially kept at a constant...

21 June 2016 6,705 2 View

Can I substitute Tween-20 with either Triton X-100 or SDS?

16 June 2016 8,949 3 View

How to compute the power output per unit area of solar PV installations?

I have computed the PV potential of certain locations and wish to determine the area of collectors needed to generate specific qty of electricity.

05 September 2013 7,232 13 View

Does anyone have experience with the following limit of the standard deviation of a set of different means?

Consider the set of 5 elements composed of two positive real numbers x and y, their arithmetic mean (x +y)/2, their geometric mean sqrt(xy), and their harmonic mean 2xy/(x + y). set = {x, y,..."> Does anyone have experience with the following limit of the standard deviation of a set of different means?

04 July 2013 8,950 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View