It is intended that the antibiotic-resistant gene replication continues to be made by agrobacterium?
Someone told me about using an agarose/RPMI mix to coat the wells and leave it to hardened before adding the cell suspension to rest overnight. Any thoughts?
18 October 2015 5,997 3 View
Hey! I aim to generate a transgenic knockin zebrafish line that mimetizes a genetic condtition that leads to a certain disease on human. To do so, I need to insert a codon for mutagenic aminoacid...
14 July 2024 6,240 0 View
Hi all, I have been facing problems with colony pcr for agrobacterium colonies. I did site directed mutagenesis, cloned to pcambia, sequenced and confirmed and then transformed to Agrobacterium....
02 June 2024 6,517 5 View
Hello all. I transformed some Agrobacterium competent cells by electroporation and performed colony PCR to see the positive transformants. However, I performed the glycerol stock after 10 days of...
26 May 2024 9,304 3 View
I am confused regarding the desired edit efficiency of PEP2 for transversion on rice plants using Agrobacterium.
21 May 2024 4,332 0 View
Dear all, I wish to measure amylin in mice plasma of C57Bl/6 mice. However, I struggle to find information in the litterature that is precise. I only could obtain one data, referencing 0.7 pM in...
20 May 2024 9,163 0 View
Strain: LBA4404 Growing media: YEB (pH 7) Temperature: 28°C, 200 rpm (overnight culture) Antibiotic use: Kanamycin and Streptomycin
13 May 2024 7,726 2 View
I could've sworn that I read a protocol where strained plant "juices" were added to agrobacterium cultures in lieu of pure acetosyringone. If you have heard of anything like this, please let me...
06 May 2024 9,001 0 View
I've recently come across the method of agroinjection and vacuum infiltration of various plant tissues (seedlings, potato eyes, tomato fruit). These methods are new to me and very fascinating but...
06 May 2024 3,036 2 View
Hello all. I purified some plasmids after doing precultures of strains from a 2-month old plate in -4 degrees. The PCR results were fine and I used theses plasmids to transform Agrobacterium...
26 April 2024 8,440 3 View
Hello, Apologies if there's an obvious answer to this, I'm a first year PhD student and this task is entirely new to me. I'm including a lot of detail to convey my question clearly. In D....
04 April 2024 9,635 3 View