Someone told me about using an agarose/RPMI mix to coat the wells and leave it to hardened before adding the cell suspension to rest overnight. Any thoughts?
A coating of your wells with agarose is a good idea. Just add 2% (w/v) agarose to basal culture medium and heat it until its completely dissolved (in microwave). Than add 25 µL to your 96wells and let it chill for 1 -2 h at RT. Cells will not adhere to this surface. After some days the cells will form aggregates/spheroids.
Many cell types will undergo anoikis due to prolonged lack of cell-ECM adhesion. This manipulation may not be advisable.
You can coat bacterial polystyrene petri dishes with F-127 Pluronics, and prevent any cell attachment. Alternatively, you can suspend cells in a DMEM/methylcellulose solution to hold them in suspension.
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