I am working on a chitosan-loaded nano formulation through ion gelation for the treatment of a specific disease in mouse model. I am planning on comparing the effect of the free drug effect on the disease with the nanoformulation.
My question is, there's already a reported dose for the free drug in the literature of 50 mg/kg, do I use the nanoformulation equivalent of this dose in the treatment? i.e. I prepare an amount of the formulation that has the equivalent drug concentration of 50 mg/kg, or do I carry out a cytotoxicity study on the nanoformulation itself and try to determine the treatment dose?
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In this study for instance. It's very similar in experiment design to the study I am about to carry out, but I am very confused in terms of the doses stated.
Thank you for your time!
Dear Mohammed,
When comparing two treatments you should alwais compare the quantity of the free drug injected and ideally the same route of administration. In your case, if you treat the animals 50 mg/kg of free drug, you should also treat your animals with 50 mg/kg of the drug encapsulated in your nano formulation. To do this, you have to charaterize well your NP to understand the concentration fo the drug in it.
I hope this helps.
Best Regards
Francesco
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