We are testing d potentials of certain marine seaweed as prebiotic candidate in diets for freshwater fish. Feeding trial was conducted to evaluate optimal inclusion levels and effects on digestibility, FCR, feed intake, PER and growth performance. We also quantified proliferation of probiotic bacteria in the small and large I intestines and ran some hematology tests.
We seek advice on particularly, some biotechnology evaluations we need to undertake to verify that the immune system of fish is boosted and that indeed, the prebiotic is a good candidate. Every suggestion is graciously appreciated
You may be interested in a paper on "Fish as Biomarkers in Immunotoxicology" describing several parameters for the function of the immune system in fish - see attach. Of course, this is not a recent paper, but could be still useful for your research. Good luck !
Hello,
You may measure plasma lysozyme, immunoglobulin, coplement activity, respiratory burst activity and bactericidal activity. You may test mucusal immunity also, by measuring lysozyme, immunoglobulin, alkaline phosphatase, protease and esterase, plus bactrocidal activity. All these parameters are good, especially for manuscript arrangement, but my opinion is that the only robust item illustrating immune status in fish is pathogen challenge.
You may be interested in two papers describing several parameters for the function of the immune system in tilapia fish - see attach. with best wishes
Thanks everyone for your contributions.
Seyyed Morteza, could you please help me attach one or two papers showing some of the suggested evaluations please?
Dr Willem, sorry the paper you attached could not be downloaded. Kindly help me attach it again or send to my email [email protected]. Thank you.
If there is anyone with a paper detailing the use of Biotechnology tools, that would be very much appreciated.
Thanks again
Usually i test the potential of prebiotic polysaccharide added in a diet for freshwater fish after 3 months of treatment
Hello, you can do the non specific humoral and cellular parameters as already mentioned above like lysozyme, myelioeroxidase,antiprotease , total globulin etc for humoral and respiratory burst for cellular. Also you can see for the enhancement of immune genes like interleukin s, tumour necrosis factor etc..plus challenge test with live vurulent pathogen
You may also use histology, to observe morphology of digestive tract and measure the enterocitys, microvillus...
Hi Andrea, Omita;
Could you mention some specifi genes that you may have tested and the expected results please?
Ricardo, what would be the expected outcomes?
Most other parameters mentioned have been checked, thank you everyone.
YOU CAN check for the upregulation of important immune genes like hepcidin, alpha 2 macroglobulin, interleukins, tumour necrosis factor etc by taking into account some house keeping genes like beta actin.
You may also check the effects of xenobiotics on cytochrome P450 and GST enzyme activities, which are easy and low cost assays.
If the fish size is large enough, you can collect the serum and run ELISA to measure the IgA or IgY immunoglobulin levels in the serum. Since the antibody is not specific, you could just measure the general immunoglobulin levels. You could also look into expression of hsp70 that relates with immune system.
The phagocytosis, lysozyme activity, complement components C3 and C4, E-C3bRR (Erythrocyte-C3b rosette rate) and E-CIRR (Erythrocyte-immune complex rosette rate) levels and circulating antibody titers in the serum.
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