In order to analyze long-term changes of oxidative stress, would it be sufficient to measure urine F2 isoprostanes in a single urine sample in humans (i.e. morning sample) or should it be measured in 24h urine?
Hi Santiago,
In my experience it's easier to get single urine samples so you do not bother the participants as much as using 24h urine. Besides 24h collection can be cumbersome and often improper or incomplete, especially in children, and it may bias the result. Then you have to standarize your parameters to creatinine, and here first morning
urine samples are very useful because creatinine is not affected by practice of exercise and food intake so it has little day-to-day variation. Thus you can choose as many time points as you require for your long-term study and compare any oxidative stress urinary parameter you want. Hope that helps!
Hi Santiago,
EFSA guidance recommend an analysis of oxidative damage to lipids by measuring changes in 24h urine sample using gas chromatography and mass spectrometry as a preferred detection mode.
I agree with Pascale. Quantification of isoprostanes intended to reflect their production for a long period, representing production chronically. Therefore, it is convenient measurement in 24-hour urine stored at 4 ° C / if possible) and supplemented with BHT to prevent oxidation of the molecules of interest. Regarding the measurement method, I also agree with Pascale is preferred GC / MS, with all its drawbacks for HOMEWORK and derivatization of samples, but is that Elisa available, in my opinion and experience, do not offer nor good practicability and good accuracy of results
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