Is it possible to use antibodies after absorption with magnetic beads?
It seems you can use 0.1M glycine, pH 2.7 and then neutralize the eluted antibodies with 1M Tris.
You must couple the peptide covalently to the beads? Then yes glycine is the method of choice. Low pH will elute the antibody, you can go as low as 2-2.5.
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
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