Translating two biomarkers to the same scale??

Hello researchers, I was wondering if anyone had experience in the following: I would like to translate two separate continuous biomarkers (A and B), that are mechanistically linked to each...

25 January 2021 2,107 3 View

What would cause different shape DMA loss modulus curve in different batches of PC+ABS?

I have some DMA curves of PC+ABS from different batches. The PC loss modulus peak is fairly consistent but one batch has a peak value 10degC lower than the rest. Another peaks at the normal value,...

06 December 2020 809 2 View

Nature of Racism?

I am writing a scholarly advocacy article - the question is: "What is the problem with using an historically specific example of racism as a template for understanding the multifaceted nature of...

29 May 2020 7,848 22 View

Phosphomimetic Peptides & Phospho-Specific Antibodies?

I am performing experiments using synthetic cell-penetrating peptides in acute hippocampal slices. Our lab has verified the NLS-mediated nuclear delivery of a number of such peptides in the region...

27 December 2019 7,914 2 View

What is the recommended fixation time of 400 um hippocampal slices in 4% PFA?

I am currently processing 400 um hippocampal slices immunohistochemically. I have not had too much trouble with my images and antibody binding. That said, I am aware that over fixation may...

22 June 2018 9,560 5 View

How long does Apex Taq RED Master Mix, 2X remain viable after opening?

The manufacturer's data sheet says the unopened vial lasts for 2 years at -20 C and that the mix is stable at 4 C for at least six months. The dye we are using has been opened and has been kept at...

10 June 2018 3,902 3 View

Is agitation necessary during IHC antibody incubation for 400 um hippocampal slices?

I am processing 400 um hippocampal slices immunohistochemically. I am incubating the slices in the primary antibody solution overnight at 4 degrees C and the secondary antibody and nucleic acid...

09 June 2018 9,562 3 View

Is it normal for signal intensity to fluctuate during chemiluminescent detection with Versadoc?

I've noticed this strange phenomenon where my signal intensity will change from picture to picture over the course of my exposure. For example: I will expose membrane to ECL for 2 min, signal...

13 February 2018 1,529 2 View

How to use McNemar's test to compare accuracy of classifications?

Hi, I have seen various studies use McNemar's test to statistically test the accuracy of several classifications. I have six classifications, produced using three different classifiers (SAM, SVM,...

19 September 2017 5,034 4 View

What is the best way to isolate total RNA (especially the small RNA less than about 200 bp) from yeast, for RNA-Seq?

I'm looking to isolate the total RNAs from yeast and then prepare them for RNA-Seq. I'll be testing the quality of the RNA with a bioanalyzer. But it seems that most options degrade the RNA or are...

25 September 2014 9,695 4 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

No title

02 March 2021 3,060 3 View

Good sites to recruit survey participants on a grad school student budget?

Any good recommendations for participant recruitment for online surveys on a grad school student budget?

02 March 2021 4,293 4 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Any research paper call for publication related to management or finance or economics?

It should be standard journal (Indexed)

01 March 2021 8,379 1 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

Centrality measures computation in Biblioshiny/ Vosviewer?

26 February 2021 7,279 2 View

How to model Supercritical water gasification (SCWG) process in Homer software?

Hi everyone How to model Supercritical water gasification (SCWG) process in Homer software?

25 February 2021 4,075 1 View

Suggestions for occupational stress surveys?

I am currently writing a masters thesis on reasons for occupational stress in the early childhood classroom, and want to include a short survey assessing participants work-related stress levels. I...

24 February 2021 8,678 2 View

Can the problems of overfitting be solved without cross validation?

For an overfit data set the cross validation error will be much bigger than the training error. Is it true or false?

23 February 2021 3,616 2 View