We would like to buy a new cell counting device for our Lab, and we're searching for a device, with a reasonable price. However, we're not sure about the reliability and the reproducibility of the results using different devices, including Logos LUNA II, Thermofisher Countess II, Biorad TC20, etc.
personally, I've used LUNA II with their 2-chamber slides and I was happy with the repeatability. Also I've used Countess II with reusable slides which was not a satisfactory experience. But I can't make a decision between the two as they were not both with single-use chamber slides.
I would appreciate if anyone can provide a head-to-head comparison, or device-to-hemocytometer comparison, or at least share their device reliability experience?
Many thanks.
No debate here! Manual counting using a Neubauer chamber (aka hemocytometer) is the only method to count cells reliably! Save your pennies and go back to classical (manual) techniques!
David Ian Leavesley Haha, that's true. However in our case, we also need to consider the scalability.
We use the Countess and for normal tissue cultured cells it works well. It does fall down a bit if there are debris, I even centrifuge the trypan blue to ensure good debris-free counts.
We are moving across to the new CellDrop machine to reduce our use of disposable plastics. The pay as you go instrument is very reasonable but it depends on how many cell counts you are looking to run.
The FluidLab R300 Holographic Cell counter is a great portable cell counter which doesn't require an additional viability dye. But at the moment it does use quite expensive glass slides.
Hello Ali Babaie ,
Although you probably already decided on a cell counter here is a paper comparing the results of a hemocytometer with an automated cell counter: Article Validation of three viable-cell counting methods: Manual, se...
Since you have not used the Luna with the reusable slides here is a result: https://logosbio.com/application_notes/using-the-luna-reusable-slide-for-accurate-cell-counting-with-automated-cell-counters/
However choosing the right cell counter for you always depends on your needs. Reliability, Throughput, Cell type, concentration matter in the decision.
Most companies offer Application notes on their websites stating the accuracy of their devices.
Comparing customer experiences on e.g. Select Science might also help.
But the best way to find the right fit is to try them out in a demonstration.
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