How to calculate MDA content from absorbance values at 532 and 600 nm?

in analysing MDA content in plant tissues, I took 0.2 g leaf tissue (fresh welght), added 5 ml 0.1% TCA and samples were grind. samples then were centrifuged at 12500 rpm for 20 minutes. 3 ml from...

16 July 2019 6,884 2 View

How can I store stool specimen for Molecular diagnosis for human parasites Is there a difference of storing for PCR or real PCR or multiplex ?

Molecular stool

08 January 2019 8,186 3 View

How you calculate the sample size for project on a clinical disease?

How you calculate the sample size for project on a clinical disease?

21 July 2018 9,389 9 View

Can somebody tell me the most common microorganism isolated from ICU?

Can somebody tell me the most common microorganism isolated from ICU?

23 July 2017 9,542 5 View

Why some planes in orthorhombic structure have four miller indices?

we know the orthorhombic structure has three axes and hexagonal structure has four axes. therefore, the miller indices for orthorhombic structure and hexagonal structure are (hki) ,(hkil)...

13 February 2017 5,956 3 View

No title

07 January 2017 7,604 2 View

Why we must modify pH value in sol-gel method ?

23 May 2016 906 9 View

Does anyone know some papers that studied thermal analysis (TGA-DTA) and (FTIR) for CaBi2Nb2O9 ceramic?

I want to these paper please .

16 October 2015 9,480 2 View

How do I test normality for two independent groups?

Hi, I am doing comparison between two independent groups; patient group of 24 subjects and control group of 34 subjects. My questions are: 1) Is testing normality relative? I mean is it sufficient...

07 October 2015 5,646 21 View

How can I correct results for a substance measured in two different machines?

10 June 2015 927 6 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Any easy method of analyzing vitamin D in foods by HPLC?

Looking for method of extracting and analyzing vitamin D in foods by HPLC

24 February 2021 7,524 7 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

What is the best method to evaluate and measure non-circulating estrogen levels in mice ?

Could anyone suggest me the best procedure to measure the circulating estrogen levels in mice ? I came across the general methods where it can be measured in serum or urine but a detail suggestion...

22 February 2021 4,352 2 View

Questio regarding micro/nano particles fabrication ?

I will start to fabricate micro/nanoparticles for growth factor release and I check out a procedure quite nice, however, it is lacking some details and since I don't have much knowledge doing this...

20 February 2021 199 2 View