I have stored my cucumber seeds treated with brassinoids at -20 °C for more than 15 days. Will there be still effect of brassinoids on cucumber seeds or I have to treat it again.
1. Brassinoids are potent plant growth promoters. A paper related to it was recently published (see below), although not directly related to your question. You can send an email to ask the senior author. They may be able to provide you an answer. [ http://www.nature.com/ncomms/2014/140819/ncomms5687/full/ncomms5687.html ]
2. Alternatively, you can take a portion of your seeds out and re-treat them with Brassinoids. You can then compare both treatments in the future to see whether any growth change etc.