Hello,
I’m doing a project about diatom and foraminifera.
I’m going to use agar/agarose plating to culture diatom. As far as I know, agar usually used for plant culture, and agarose for protoplast. Moreover, I know that Kei Kimura & Yuji Tomaru (2012) have published a unique method for culture diatoms.
But what’s the difference between agar and agarose plating to culture diatoms?
Hope to get some answers and suggestions.
Thank you!
The major difference is the purity, agar has a lot of pectin and I think is more acidic. So it really depends upon the organisms and its tolerance. Agar is much cheaper but you could in theory use agarose pretty much anytime it calls for agar (but not the reverse).
Agarose is purified form of agar. Agar is somewhat translucent than agarose. Preferably, agar is used for culture of microbes including diatoms. Moreover, agar is a cheaper option while both agar and agarose act as gelling agents.
I am working on diatoms culture . 7 gr Agar for 500ml with pH 6.
picture attached.
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