What's the best protocol for using A23187 ionophore in calcium imaging to convert fluorescent intensity to calcium concentration(using fluo 4 AM dye)?

14 December 2020 0 9K Report

I have a problem for loading cell in A23187 calcium ionophore to achieve Equilibration of intracellular and extracellular Ca2+ concentration.

Badges
Science topic

More Afsane Bazzi's questions See All

Deadly does of wood vinegar ?

How much is the deadly does & food toxicity of wood vinegar ?

25 December 2023 4,773 0 View

Is wood vinegar potentially toxic to insects and bees?

03 September 2023 351 2 View

Can we calculate the theorical maximum adsorption capacity of an adsorbent before doing the experiment?

Hello dear researchers, If we have a known adsorbent and a known adsorbate, and each single entity of the adsorbent is supposed to be an active site of the adsorption. The molar mass of the...

08 December 2022 8,823 3 View

How to determine tidal elevation changes correctly in MIKE 3 FM?

Hi. I am working on my thesis with MIKE 3 model in Persian Gulf . For validation i compare measured water elevation changes with simulation results by changing bed resistance. The problem is the...

11 November 2021 1,544 0 View

How can i analysis result of gas absorption in gromacs?

Hi every body I want to simulate gas absorption with gromacs. I have a box with three part , amine and water in the middle of box and gas is around of it(gas+amine solution+gas). Firstly I...

02 October 2020 492 2 View

How do EN15662 extraction salts work?

Hi. Does anybody know how EN15662 salts can be useful to extract?

15 May 2020 5,324 0 View

THRESHOLD for E GENE COVID 19?

I am trying to define the threshod of COVID 19, E GENE detection using ABI 7500. WE are using KOREAN KIT powercheck however, the THRESHOLD is not define in the package insert, the FLUOROPHORE is FAM.

22 April 2020 5,434 3 View

Where is solvation model (SM8) in gaussian ?

I want to find a correct charge distribution of amines . I found that solvation model (SM8) has correct charge distribution .how can I use this solvation model in gaussview and gaussian ?

10 February 2020 4,510 4 View

How can I calculate the cortical thickness and different brain volumes of segmented MRI images ?

I have 3T, T1- weighted, MR images of marmoset brain. I have segmented these images into GM, WM and CSF (using SPM). My aim is to calculate the cortical thickness and the brain compartment's...

19 March 2019 1,044 4 View

Why Isotherm Absorption has been zero In the adsorption process of ethylene (or butane) on polyethylene?

I study the sorption of ethylene(or butene) on poly ethylene, i use the materials studio 8.0, in build polymer ten repeat unit was selected , in first geometery optimization was done then the...

22 October 2018 3,208 3 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under op microscop?

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under optical microscope?

07 August 2024 2,132 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds?

XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds

04 August 2024 3,019 3 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

How to calculate the molar proportions of the oxides from the XRF analyses?

02 August 2024 2,565 2 View

How to correctly use the UTE and ZTE pulse sequences in Bruker's ParaVision software?

I am using a Bruker 600M solid-state NMR spectrometer with a Micro 2.5 microimaging system. The test sample is a tube of 1M LiCl aqueous solution, and the nucleus detected is 1H. I am trying to...

01 August 2024 9,227 1 View

Looking for help on sem image analysis?

Hello I am conducting a microstructural analysis of a soil treated with lime. The following sem images are of the untreated s1 and treated soil s3. The untreated soil contains quartz calcite...

01 August 2024 572 0 View

How to find projects for a team of professional developers in AI?

We are looking for projects for a team of professional developers About us We are a team of experienced developers with over 10 years of experience in various programming fields. Our mission is to...

30 July 2024 8,377 0 View