We're having trouble extracting DNA from ruminal fluid samples we used for in vitro testing. The in vitro trials we are performing require the addition of salts, which we think are interfering with the extraction process.
We performed a literature search, but did not find the answers we are looking for.
Which kit are you using for ruminal fluid + buffering salts?
There are several commercially available DNA extraction kits that can be used to extract DNA from ruminal fluid samples obtained from in vitro trials. The choice of kit depends on various factors such as the volume and type of samples, the downstream applications of the extracted DNA, and the laboratory's specific requirements. Here are a few commonly used DNA extraction kits that you can consider:
1. QIAamp DNA Mini Kit: This kit by QIAGEN is widely used for DNA extraction from various sample types, including fluids. It utilizes silica membrane-based technology and provides high-quality DNA suitable for a range of downstream applications.
2. PowerSoil DNA Isolation Kit: This kit by MO BIO Laboratories is designed for DNA extraction from environmental samples, including microbial DNA from ruminal fluid. It utilizes bead-beating and column-based purification methods for efficient DNA recovery.
3. PureLink Microbiome DNA Purification Kit: This kit by Thermo Fisher Scientific is specifically designed for DNA extraction from microbial-rich samples such as ruminal fluid. It combines chemical and mechanical lysis methods for efficient DNA isolation.
4. ZR Fecal DNA MiniPrep Kit: This kit by Zymo Research is optimized for the extraction of DNA from fecal and intestinal samples, which may also be suitable for ruminal fluid samples. It utilizes bead-beating and column-based purification methods for DNA extraction.
These are just a few examples, and there are several other DNA extraction kits available in the market. It's advisable to consult the product specifications, user reviews, and published literature to determine which kit best suits your specific experimental requirements and budget.
Have you tried to freeze-dried samples before extract DNA? Sometimes may help to extract DNA in tough samples. Phenol-chloroform extraction with a bead beating step is usually effective, although not recommended because of the toxicity of this chemicals.
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Carlo
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