Hello
I am trying to obtain chitin from crab. I haven't neutralized the product of deproteinization step but I have spent a lot of water. Is it necessary to reach a 7 pH before starting demineralization process? How could the product be affect?
If the pH value is not neutral, the demineralization process could be incomplete.
Dear All: I have to quantify the fluorescence intensity of cells that are fluorescent to two different markers, so i'm interested in finding out if this is possible. Previously I have...
22 July 2024 8,770 1 View
Does anyone know what is the refractive index of MoSi2 and in what medium it can be performed (distilled water or ethyl alcohol)? I pretend to perform a particle-size distribution analysis by...
23 March 2024 6,736 1 View
Apart from the size I can´t find any information about the LFP batteries used in BYD ev, for example. Can the size affect the discharge of tha battery or other characteristics?
19 March 2024 2,229 1 View
Con esta pregunta se pretende identificar los métodos y técnicas más adecuadas para abordar las razones por las que las personas drogopendientes totalmente rehabilitadas, se encuentran en...
10 March 2024 6,561 2 View
Hi! I ran different models using the glmer function in the lme4 package and compared the performance of these models using the compare_performance function in the performance package. The model...
06 March 2024 281 1 View
I would like to know if it is possible to use antibodies (in this case I would like to use two markers of neutrophils and monocytes such as FITC anti-mouse Ly-6G and FITC anti-mouse Ly-6C) whose...
04 March 2024 9,943 3 View
Actually, I am trying some culture mediums adding different concentrations of PEG, simulating different osmotic potentials. The objective is to evaluate germination and growth on primed seeds in...
10 January 2024 2,961 3 View
Hello everyone. I am struggling to get good quality results on my Sanger sequencing reactions. I don't know the specific problem that I am facing. In a single order, most of the chromatograms...
03 November 2023 4,035 0 View
Since retinal is a photosensitive compound, I wonder whether you perform your experiments in darkness or do you allow certain amount of light. I've read some experiments for root development and...
15 October 2023 4,863 0 View
We have made almost 400 laser experiments where the measurements are done through a photocell, registering voltage variations corresponding to variations in the intensity of the laser beam. When...
02 October 2023 7,538 2 View
How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under optical microscope?
07 August 2024 2,132 3 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds
04 August 2024 3,019 3 View
Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope...
02 August 2024 7,843 0 View
Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope of...
02 August 2024 8,572 0 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Hello I am conducting a microstructural analysis of a soil treated with lime. The following sem images are of the untreated s1 and treated soil s3. The untreated soil contains quartz calcite...
01 August 2024 572 0 View
I've attempted to use this method to synthesize copper nanoparticles. Copper nanoparticles can be synthesised using a variety of precursor materials. CuSO4, distilled water, NaOH, and EDTA are...
28 July 2024 8,027 3 View
Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...
22 July 2024 9,761 5 View
My research work is related to the calcination of limestone. I have a complex question and would like to consult with experts in this field. Could you please advise which kiln to choose (a...
18 July 2024 1,390 2 View