I'm trying to insert EGPF into a plasmid vector (PTP6). the source of EGFP is another plasmid vector pEGFP-C1). In order for me to excise the EGFP from the vector I have two options : one is using AgeI and BsrGI, which will result in giving me a EGFP that is three amino acids short ; the other option is using AgeI and XhoI, which has a restriction site in the MCS of that vector and will give me an additional four amino acids right after the EGFP. What will happen if I used BsrGI?. is it going to affect the ability of EGFP to produce fluorescence since we lost three amino acids ?.