Will there be anything called the dominant negative effect
I am facing difficulties to insert the pile in soil layer and define the pile exactly through Flac-3D code.
22 June 2024 8,334 1 View
Respected readers, I am working with a totem pole PFC circuit. In an experimental study, I face a problem of high-frequency notches in source voltage (Yellow one). I found an explanation behind...
14 May 2024 6,692 6 View
Davenport wind spectrum can calculated through software, but I couldn't understand how it is implemented on a structure through software application. if anybody could enlighten me, it would be a...
05 May 2024 9,728 3 View
We have grown boron nitride with a monolayer thickness. XRD usually is used to understand the polymorphism of any material. However, due to the minimum thickness of the sample, it won't work for...
15 April 2024 5,431 1 View
I have deposited chrome on the glass wafer. Then I applied a dielectric layer of SU-8 over it. After that to make the surface hydrophobic, I applied Teflon by using spin coating. However, I found...
12 April 2024 7,009 3 View
To prevent intestinal disorders, probiotics can be rendered multidrug-resistant to survive in the presence of co-administered antibiotics, and they may also transmit the antibiotic-resistance gene...
01 April 2024 8,043 2 View
Hi everyone! I want to isolate the PBMCs from AML patients and transduce the cells with my lentivirus to knockdown a protein and use for further experiments. I am unable to find a single widely...
14 March 2024 4,317 0 View
How to plot Raman mapping data in origin software?
27 February 2024 9,522 0 View
is wind spectrum consume mean wind and turbulence both ? and how the mean wind will be calculated from measured wind data?
04 January 2024 8,546 2 View
What methodologies are involved in sequencing and aligning the genome of a mosquito species for which the sequence is not available in the NCBI? Additionally, how can one construct a phylogenetic...
19 December 2023 6,721 0 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
06 August 2024 726 3 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
