We have a Pseudomonas sp. isolate which turned brown on PDA and make whole plate brown within 5-7 days with strong anti-fungal activity. But it does not change brown on LBA, TSA or other media.
First one on kings-B and other on PDA
This could be possible due to two reasons. Either the strain is secreting some kind of secondary metabolites or may be due to differences in the surroundings that may cause oxidation.
Many reasons can lead to formation of such colour in PDA. Production of melanoid pigment and or one derivative dopa. It could be a reason of this.Please review the conditions the organism can produce dopa . This organism could be tyrosinase positive Anyhow try to read a literature on formation of melanin pigment by P. aeruginosa , Dopa formation. Tyrosinae activity by P aeruginosa. Also look to
Lawrence C Parks 1997. Atlas R M Handbook of Microbiology Media 2nd Edition.
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Hi Satish
You can find some useful references 1
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4699791/
6
https://ijbiotech.com/article_6981_0cc9faa7a2cc1e039f9a855faa2c6b8c.pdf
70
7
https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=9&cad=rja&uact=8&ved=0ahUKEwivzr7xqYvUAhUHPhQKHREJDcQQFghcMAg&url=http%3A%2F%2Fyadda.icm.edu.pl%2Fyadda%2Felement%2Fbwmeta1.element.baztech-2310c708-ebd9-4215-9f5b-1eef3b9443c7%2Fc%2FPECO_2013_1_part1-9.pdf&usg=AFQjCNEQ5QLGsS4Jm_LrTdUfcD4LWBZ3mw&sig2=KVDBEMYnX1dfSRRdx_GJqQ
Thank you Dr Laith Al-Ani for useful references. I am looking for some primers related with antifungal metabolites of Pseudomonas sp.
Thanks to Dr Amit, Gamal Enan and Sosanka PROTIM Sandilya for your suggestions
satish
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