Association constant can easily find out from the UV-visible spectroscopy for 1:1 inclusion complex.... But would the Benesi-Hildebrand equation & corresponding calculation be the difference?
Most of the peoples had read this question.... But benefit?? No one didn't share any answer!!
Is not research a collaborative aspect of academic life??
Hi Raja,
Do you mean 1:2 when ou write 2:1 (please give an example) because your question is too vague and give no infos for a valuable answer.
A good formulated posted question will share a lot of answers.
Please read the paper :
http://www.sciencedirect.com/science/article/pii/S1872150807600710
Abstract
Benesi-Hildebrand (B-H) method is a widely used approach for determining the stoichiometry and equilibrium constants of nonbonded interactions, particularly 1:1 and 1:2 interactions. Using computer simulation, it was shown that, under certain conditions, the approach could generate inappropriate stoichiometric conclusions for 1:2 interactions. This problem could occur in the cases of both weak and strong interactions, where the 1:1 B-H plots showed a linear feature and the 1:2 B-H plots showed a nonlinear feature. In addition, effect of the initial concentrations on the accurate evaluation of equilibrium constants of 1:1 interactions was investigated. It was found that the minimum safe concentration ratio r0between ligand and central species was 100. However, for weak nonbonding interactions, for example K91%. The other inequation, KC 0B>0.1, was not found to be the safe condition to validate the B-H method.
Key Words
Benesi-Hildebrand method
Molecular interaction
Equilibrium constant
Stoichiometry evaluation
Good luck
JRG
Thanking you Sir.
Usually, we are mean Host: Guest ratio by mentioning such kind of ration!
Here I wanted to mean Host: Guest ratio is 2:1.
One more thing is that nonlinearity should persist for both 2:1 & 1:2, if it actually exist
! Right Sir Jean Rene Grezes ??
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