I am planning to map QTLs for drought and it's related traits in maize using SNP's. Want to know What would be the minimum or optimum, mapping population size for QTL identification for the drought trait / any other complex trait in maize. The number of plants per line @ F6 generation? On what basis we will say we require 250-300 population size?
Hello Anand,
The size of your experimental population can be calculated starting from the standard error of differences between two means (for the trait of interest, in the parental lines that were used to obtain the respective experimental population).
You can find practical details in the tutorials from "R/qtl: A QTL mapping environment. Software for mapping quantitative trait loci in experimental crosses", here: http://www.rqtl.org/. R/qtl allows you to run simulations with different types of populations.
Also, Helmut van Emden, in "Statistics for terrified biologists" (Appendix 1, page 306) explains the principles in a very biologist-friendly language. You can find the pdf here: http://www.jncasr.ac.in/chronobiology/reading%20material/Statistics_Terrified_Biologists.pdf
Hope this helps and good luck with your experiments.
Dear Sir,
Thank you for your kind response, I think two links provided in above text will be useful. I will go through them get back to you any thing further requires.
Regards
Anand
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