For the enzyme Tsp45-I...
I want to work on PBS in molecular breeding and want to know are their any genes or tightly linked markers available for PBS in Corn. I want use these in our breeding programme.
10 November 2018 1,585 2 View
We are producing the double haploids in Maize and we need to identify the double haploids in early stage of the plants. I want to know what are the best and accurate method for identification of...
10 November 2017 5,640 18 View
I am in plan to develop sweet corn from our elite germ plasm lines using the sweet corn genes Su1, Su2, Se1, Sh1, Sh2 and bt1, bt2. In this regard which genes combination will be better. Eg:1. Su1...
05 June 2016 1,542 9 View
I am planning to map QTLs for drought and it's related traits in maize using SNP's. Want to know What would be the minimum or optimum, mapping population size for QTL identification for the...
05 June 2016 1,223 2 View
Corn Rf genes?
01 February 2015 3,327 2 View
I want to know the better or best way of analysis of data and scoring of markers. I am analyzing the SSR marker data in Tomato for genetic diversity. I have scored the marker data with their base...
05 June 2012 7,381 14 View
I want to use SSR markers, I think these will be good for hybrid purity checks in vegetables (Cucumber, Brinjal, Chili and Tomato) and I am also looking for the sources for SSR markers in...
03 April 2012 5,509 42 View
I have some primers which i need to screen for different diseases, these involve the TETRAARMS-PCR procedure. Can anyone offer advice?
02 March 2012 5,113 0 View
I am doing restriction digestion for TSP45i and ScrfI enzymes with PCR product of plant genomic DNA . Not able to, differentiate between before and after restriction digestion..
31 December 2011 3,471 6 View
Does anybody have any ideas regarding these?
31 December 2011 5,521 6 View
I'm very interested in biotechnology and medical research and I'd like to meet a professional here and talk about it together
28 July 2024 3,798 4 View
List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?
27 July 2024 4,800 4 View
I am pleased to announce the CUSABIO Biotechnology Scholarships for Students 2024! This scholarship aims to support outstanding students pursuing studies in the field of biotechnology. Here are...
22 July 2024 5,790 1 View
I was wondering if it is possible to form a permanent open "ssDNA bubble" similar to a transcription bubble (>13 nucleotides) within E. coli. These criteria are important: 1. Open ssDNA...
07 July 2024 8,275 5 View
Give in detail with available literature and website links for submissions
27 June 2024 9,753 1 View
Hey everyone! I'm having difficulty marking the C4 complement factor in Western Blotting. Has anyone had contact with this antibody or made it in-house? I have already used Anti-C4β (D-12)...
17 June 2024 6,124 1 View
Generally for bacterial DNA isolation we use a fresh culture of 2-5 days old. But if we use an older culture say 10-15 days old will it have any impact on the DNA content and the DNA isolation...
13 May 2024 3,061 11 View
I was curious as to why modern soybean half-seed protocols use 1/10x strength B5 salts during the co-cultivation step rather than full strength B5 salts? Also, are the B5 vitamins added at full...
07 May 2024 3,924 0 View
I am following the standard method which is mentioned in Tomotake Kanki et al., 2009 research paper Monitoring mitophagy in yeast: The Om45-GFP processing assay . I am growing the cells in SD-HIS...
04 May 2024 6,463 0 View
How are these exploited in biotechnological applications?
07 April 2024 566 5 View