I am looking at the intrinsic quenching of tryptophan residues in a protein by coenzyme A. The curvature I got is not linear nor upward, but instead ressembles a nonlinear plot of michaelis menten kinetics. From the data plotted of Fo/F versus quencher (coenzyme A) concentration, I want to get a binding constant. What would be the equation to do this? Thank you.

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