Isabella A Iasenza

5 Questions 8 Answers 0 Followers

Questions related from Isabella A Iasenza

Gating and compensation for Annexin V and PI?

My questions are the following: 1) I first have my FACs profile, SSC-A vs FSC-A, I then have two plots that follow to remove doublets (FSC-W vs FSC-H, SSC-W vs SSC-H). I wanted to know if my P1...

27 July 2019 8,532 3 View

How to enrich for CD34+ population from a cell line (without cell sorting)?

I wanted to know if enrichment kits can also be used for cell lines or if they can only be used for buffy coat and peripheral blood samples? Thank you!

29 January 2019 7,403 3 View

What would be the equation to use for a nonlinear stern volmer plot?

I am looking at the intrinsic quenching of tryptophan residues in a protein by coenzyme A. The curvature I got is not linear nor upward, but instead ressembles a nonlinear plot of michaelis menten...

20 July 2018 6,419 4 View

What concentrations to use for receptor (protein) in fluorescence polarization when KD is not known?

I am trying to determine the binding between a protein and cofactor, however the KD value cannot be found online/published. Would the concentrations of my receptor I choose for my assay be more of...

11 May 2018 6,073 5 View

K562 cells marker expression

Does anyone have experience with the markers expressed on K562 cells? I have checked DSMZ and when I stain my cells with the markers stated that K562 should be positive for and run the profile on...

01 January 1970 609 3 View