How to find a correlation between coordinates in a curvilinear coordinate system?

Hello. I have a skewed elliptical coordinate system (I use alternative elliptical coordinates). And I need to determine connections between coordinates in order to fetch a covariance matrix of...

28 February 2021 364 3 View

Failed Chromatograms on HPLC-ESI Orbitrap?

Hello fellow researchers, I recently have a problem with an HPLC coupled to an Orbitrap mass analyzer. I usually use some protein mix to make sure the equipment is ready to separate and read...

09 February 2021 3,595 8 View

Do you know any available open-source data on animal behavior for model validation?

In my research related to monitoring the cow behavior with help of sensors I have a permanent and principal problem with the data for model validation. I tried to find some available open-source...

26 December 2020 8,197 2 View

Do you know of research in communication/media studies using "Activity theory" ?

Hello, For a project which studies how teens deal with social media and their interfaces, I'm interested in so-called "activity theory" (also know as cultural-historical activity theory). I know...

21 October 2020 2,364 4 View

Northern blotting advice?

Hi everyone! Does anybody know about a good website or place to get some guidance on Northern blotting? Also, advice on blotting the RNA ladder?

07 October 2020 739 3 View

Did somebody tried to use the attR1 and attR2 sites without the chloranphenicol resistance gene i.e. AttR1-ccdB-AttR2?

I am trying to design a vector as compact as posible to test DNA transcriptional enhancer and/or repressor activity, I already have a compact bacbone (pICOz vector), minimal promoter, translation...

22 August 2020 1,083 1 View

How to amplify phage DNA (dsDNA) for producing restriction profiles?

Hi everybody! I need to amplify phage DNA so as to be able to produce restriction profiles using different restriction endonucleases. Can anyone recommend which primers should I use? I know random...

06 August 2020 1,194 3 View

Is there any method for tuning Subspace Predictive Controllers (SPC)?

My current research is about SPC and I'm interested in tuning strategies for these types of predictive control approaches. The cost function is on the photo and the tuning parameters are Np...

23 July 2020 1,176 1 View

Some TWIP steels are susceptible to hydrogen-induced failure, but adding up to 2% aluminu improves the situation. What mechanisms explain this phenom?

On the role of aluminum in TWIP steels

22 July 2020 5,670 4 View

Do you think the teacher should switch roles with the students while teaching a foreign language through TIC?

There are some theories, as Pelgrum and Law (2003) suggested that it is necessary to make a switch between the teacher and the student roles. I mean, the student should be also the active role in...

15 July 2020 5,169 15 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View