Hi Vivek, 

I've been reading and it seems that this method works for E. Coli. Maybe you could find some references inside to see if it can be applied to other strain.

https://www.ncbi.nlm.nih.gov/pubmed/10829079

This one was reported to work for Pseudomonas aeruginosa:

https://www.ncbi.nlm.nih.gov/pubmed/11111024

Also, there must be probably some CRISPR KO technology development going on for bacteria, so try to look for them.

If you KO one of the subunit of a complex, probably you will have degradation of the other subunits, but sometimes it's not the true, so if a simple KO doesn't work, you will probably have to KO all of the subunits.

Cheers,

CG

Thank you Carlos for sharing the necessary information. Once the work is successful i will share the methodoly.