How to purify cycle sequencing PCR reaction?

i am purifying cycle sequencing PCR reaction. i have tried multiple methods given in BDT manual (provided with the instrument) .everytime i used 96 well reaction plate for this purpose. 7 wells...

14 February 2021 8,534 2 View

Does any one have experience with Autoclavable cell culture media (RPMI 1640/DMEM)? Does it work well?

12 February 2021 2,958 1 View

What are the reasons of asymmetrical bilateral knee Osteoarthritis (OA)?

Primary osteoarthritis - is degenerative joint disease without any apparent underlying cause then what are the reasons of asymmetrical bilateral Knee OA?

09 January 2021 7,524 8 View

Zero normal flux boundary condition for shallow water equations?

I am looking for any book/article reference about the mathematical description of zero normal flux boundary condition for shallow water equations. My concern is that for a near-shore case how it...

16 December 2020 9,383 8 View

How to fix the fungus for SEM imaging?

I want to perform the SEM of candida sp. and i want to know that the fixation for the bacteria and fungus are different or same?

10 December 2020 6,169 3 View

How to adapt a mature Questionnaire?

I am looking forward to seek some help regarding adaptation of mature questionnaires. I am new to quantitative studies and wants to use some demographic in my questionnaire, is it necessary to add...

08 November 2020 7,146 5 View

Primer degradation.....what could be the possible reasons for not having PCR results.????

HI! whenever I start doing PCR with a new primer pair that is lyophilized.1st I make 100pmol solution then 10pmole dilution of it as a working solution. I got good results with this freshly made...

01 September 2020 2,114 15 View

Which are the best open source plotting software?

Dear friends, Please suggest few good graph plotting software that are freely available. If it has an contour plotting option, it will be an added advantage. Origin is a paid software which is...

06 July 2020 9,106 15 View

How can I import a user subroutine into Abaqus component in Simulia Isight?

For optimization my material parameter I use Isight software. I have an input file that simulates the material model via UMAT subroutine. How can I import this inp. file in the Abaqus component of...

14 May 2020 3,552 3 View

Name the hydrological models, which can simulate ice dynamics?

Please explain their principles briefly with references and flowchart. What are their advantages and limitations?

12 April 2020 2,718 1 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View