I have to take absorbance of nano particle solution. For this which solvent is recommended to use as a blank? Is it only silver nitrate solution or anything else?
Md. Shoebul Islam
You are studying not a solution of nanoparticles, but a dispersed system of nanoparticles in water. To do this, it is necessary that the particles do not settle. If the spectrum is in the visible region, then you will see a band of plasmonic absorption of nanoparticles of silver, gold, platinum metals. It is not clear why you need to measure water absorption in this case.
Md. Shoebul Islam For a blank (e.g. in a dual beam spectrometer) you need the particle-free continuous phase within your system. This continuous phase (with soluble surfactants, additives, plant extracts and so on), CP, can be obtained by filtering off or centrifuging the particles out of the dispersion retaining some of the starting dispersion for measurement. If this is not convenient (e.g. you lose your entire production this way!) then this CP can be obtained by using the (clear) plant extract before you add your precursor nanoparticle solution and any other reducing agents.
I think what you need to do is to scan for the wavelength at which your nanoparticles suspended in the plant extract absorb maximally. This should fall within the range of the characteristic surface plasmon resonance (SPR) for the nanoparticle of interest.
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