I have a company product of Biofertilizer which has Phosphorus solubilizing bacteria (Bacillus polymyxa and Bacillus magetaruim) now I want ti find out active bacterial concentration in this product, what will be the procedure ?
The initial isolation of bacteria from the soil you may do by dilution plate technique after plating on PKV agar media. Then the PSB concentration after incubation of specific time (normally 5-7 days) you may measure using liquid media of PKV and measuring the optical density after using spectrometer.