Many models are developed for validation of active principles or single component, but this can not be said for poly herbal medicines.

I think mainly studies on laboratory animal models may be tried for that purpose.

Pattanayak is right.

Hi, what did you mean by validation multi component method? Did you mean validation method of analysis? or validation of the bio-activity? or both of them? I think you can do both by performing metabolite profiling (LC MS MS or NMR) and bio-activity studies using in vitro or in vivo methods. By using MVA (PCA, PLS-DA etc) you can predict the bio-active or toxic compounds from the herbal preparations.

Chemical analysis of herbal drugs should be performed by marker and profiling approaches and both of these should be validated.

Although its very difficult to do the validation of multicomponent herbal formulation. But if you fix the need it would make easy for you to validate.

It will be more intereting to tell that collection of the drug may varie its component e.g. % alkaloids, carbohydrates, glycosides etc.

Age of the plant also affect in the chemical constituents.

And many more factors

It was just about a single plant, no of variance will be increase with no of plants (attribute).

So either u need to fix a pharmacolkgical model and according to that fix the chemical moiety.

Some time ratio of herbs may also vary the effect.

General Ayurvedic Formulation Triphala 1:1:1(Amlaki:haritaki:vibhataki) is good as laxative while its 1:2:4 decoction used in cleaning of eyes.

So, Multivariate Analysis or Principal component analysis(PCA) will help if u had design ur work from the begining and you know what is Design of experiment(DOE). After DOE it become easy to use PCA and it will become easy to get a conclusion.

Hope it Works.

The problem with 'herbals' has been defining the active ingredient (API). This is no problem with pharmaceuticals which has an IND (API), as they go through clinical trials and cure/mitigate a known medical condition.

The question then is 'herbals' cure what? What do you validate? What is the purpose of this validation?

Dear researcher,

Validation is big term. Which type of validation you want? And for which purposes? Method validation /process validation /drug validation? What?

According to your need, you will to choose the method..

Thanks and best luck.

Are you developed analytical method for same?

Please explain it. Then only we think about validation. And what type of method according to this validation parameters are selected

Are you done isolation of active constitute?

Are you use markers for identification or any instruments like NMR, IR, MASS etc

Which method you developed? HPTLC, HPLC ...etc

So according to you desicide

If concern validation follow ICH guidelines

Validation of polyherbal formulations can be done via fingerprinting utilizing LC MSMS where distinct chemical entities can be identified and then later isolated via other techniques.