I did a water-soluble extract from a fruit and I'm going to use this extract to treat cells in vitro.
In order to test the highest dosage in vitro (2 mg/mL), I will need to use a large volume of extract dissolved in the medium for the treatment. What is the maximum volume I can use in vitro (dissolved in culture medium) which will not disturb the cells and cause their death?
Initially, I put 30 ul of extract in a total volume of 150 ul (20% of extract in medium). Is that too much and eventually can cause cell death? Does anyone have that experience?
Thank you.
If at lower concentrations it had no effect, I always add a solvent control at par, in this case you could use sterile water if your extract is aqueous and do the viability test and the result would show that the extract is the one with the activity.
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