Should I show WB bands of protein's full-length and cleavage in single blot image (saturated full-length) or in each seprated images (unsaturated)?

Dear All, I have question on choosing WB bands image for publication. My target protein has two forms: 1.full-length form (very strong signal) 2. cleaved form (faint/low signal) in the same blot....

04 May 2016 7,202 5 View

How can I optimize blush-like/blur? Immunofluorescence (IF) image quality to be more dot-like?

I have done IF with Abcam antibody, but the image that I get is not look like as the Abcam review on the same anitbody. My image (attached red image) is kind blush-like or blur color?, but from...

04 May 2016 8,103 3 View

Is there a way double-color immunofluorescence staining for two different protein, but have same rabbit host species antibodies?

Dear All, I tried to study co-localization of two different proteins by using immunofluorescence staining, since the western blot result of both protein shows significantly increase in nuclear...

03 April 2016 1,461 10 View

Problem with cell deteaced/morphological changed after fixation with 4%PFA, How should I optimize the protocol?

Dear All, I have SH-SY5Y grown on coverslip (precoated with 0.01mg/ml for 1hr at 37C) in 24 well plate. When the cells were ready, I wash it breifly with PBS, then fix it with 4% Paraformaldehyde...

02 March 2016 7,594 18 View

Why FasL protein expresses in western blot lower in H2O2-induced cell death (compare to Ctrl)?

Dear All, I am study FasL protein expression using western blot in oxidative stress-induced cell death in SH-SY5Y. My results mostly shows that FasL expression is non-significant changed or...

02 March 2016 8,956 4 View

What is different between RM or Ordinary one-way ANOVA & What kind of experiment is suitable for each of them?

Dear All, I just switch from SPSS to Graphpad prism 6. In analisys, there is option to select experimental design between RM (repeated measure) and Ordinary (no pairing) for one-way ANOVA. What is...

01 February 2016 3,821 4 View

Is Fas ligand's western blot signal strong in general cell line?

01 February 2016 7,443 2 View

How should I select western blot band to measure if getting multiple target protein bands? any online database to look for?

Dear All, I am real newbie in protein field. I would like to ask you a few question regarding to select band to measure in western blot. In case that blocking is fine and I use single primary...

01 February 2016 1,304 8 View

How should I solve blochy/uneven background problem on western blot?

Dear All, In my western blot experiment, I have problem with blotchy blackground (like in attachecd picture) on the blot, which usaully appear when I expose low signal band for 15-30 min or more...

31 December 2015 3,101 7 View

How can I analyze a western blot to determine significant difference of a target protein that has no band in Ctrl, but shows band in condition group?

Dear All, I have a problem to do stat analysis to determine significant difference between control group and each condition group (vary dose of H2O2 treatment to cell line) of a target protein in...

11 December 2015 4,456 13 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View