We are working on repurposition of drugs against Japanese encephalitis infections by using computational techniques. Further, we would like to check the antiviral potential of screened drugs under in vitro conditions without using viruses such as protease assay etc. Kindly suggest more assays.
Thanks in advance.
It is logically impossible to check the effect of a compound on a virus without using said virus. However, if you know the target (enzyme) that the drug is directed against, you could measure inhibition of that enzyme, using a transgenic enzyme. Such data could at least justify the expensive use of a BS3 or BS4 laboratory for the real test.
In your case you need to know what is a target for the drug: viral RNA or viral proteins Das the drug binds to these viral components selectively? Can you check it first?
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