Alternative method of Permeablization in IL17A assay by flowcytometry?

I am evaluating Th17 cells by staining stimulated cells with IL17A and IFN-Y antibody by flow cytometry. Permeabilization step was done with Cytoperm/cytofix reagents (BD Bisciences) yet and the...

01 December 2020 4,553 1 View

How to use commands in ansys workbench?

I am trying to input the material data in ansys mechanical with the help of command i have.This experimental data only consists of stress strain value of concrete cubes.I dont know where this code...

02 August 2020 7,769 5 View

How to apply displacement boundary condition in ansys?

i have data of compression test conducted on concrete cube of 150*150*150 in form of failure loads. How to calculate the displacement to be applied as a boundary condition in ansys workbench if...

07 May 2020 8,149 4 View

How do I add a particular RF Switch to CST Simulation?

I have a particular RF Switch with me that I need to test on CST. Is there any way to import the data of that particular RF Switch (such as equivalent circuit and/or S parameters) and test my...

22 September 2019 5,063 1 View

Can anyone help me find target displacement of high rise structures with and without core and outriggers?

I went through FEMA-356 and FEMA-440 for target displacement and I cam across the formula as well but it wasn't helpful as i'm not able to understand it completely and I wasn't able to find all of...

19 September 2019 9,261 5 View

How to learn modeling engine and transmission in LMS Amesim easily?

I am totally new with the software and want to develop engine and transmission as well as complete vehicle Model in AMESIM. Also is it possible to carry out 1D CFD Simulations

26 August 2019 1,843 4 View

How to perform amino acid conservation analysis of Dynein heavy chains??

I have dynein inhibitors, and want to prove that they inhibit the dyneins of Leishmania (my model organism of interest). I would like to perform an amino acid conservation analysis where I can...

28 November 2017 2,704 2 View

Need suggestions to deal with problem of rising royalty payments in India.

Is the restrictive cap only way out?

06 July 2017 5,175 0 View

How to perform Malachite green assay for studying ATPase activity??

I am trying to measure the amount of inorganic phosphate using the malachite green assay (Carter SG and Karl DW. 1982. J Biochemical Biophysical Methods). They use a sodium molybdate solution...

01 March 2017 5,706 9 View

What is the volume of a single Leishmania cell?

Can anybody suggest any references where the cell volume of Leishmania promastigotes has been measured?

15 May 2016 2,206 3 View

Physics: Why do we describe light by referring to its wavelength, but microwaves and radar pulses by referring to their frequency?

In physics, the description of the properties of light generally refers to its wavelength (nm), while in the range of microwaves and radio waves we refer to the frequency of a signal (e.g. kHz or...

03 March 2021 1,682 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

What is Flat Lens Antenna?

28 February 2021 5,433 3 View

Can impedance of antenna be less than 50 ohms?

I am simulating helix antenna in hfss, right now, giving excitation with 50 ohms port. When I see the results of Z parameter (real). It shows lower than 50 ohms. Do not understand, if it can be...

27 February 2021 4,218 7 View

Gas sensor response and recovery time independant of flow rate and chamber volume?

I am trying to obtain the response and recovery times for my gas sensor device. It appears the the response/recovery times are primarily dependant on the flow rate of the gas I inject into the...

26 February 2021 1,511 3 View

Open Source IoT Simulators supported by Machine Learning?

Dear Research Gaters, Let me first add context to the question. I am looking to simulate the waste management system and resources of a city. The hardware devices I need to simulate include,...

21 February 2021 2,333 4 View

Could the "incoming radiation (net radiation)" values be negative?

Could the "incoming solar radiation" values be negative? What is the difference between "global solar radiation" and "incoming solar radiation"? Which one might be measured negative values?

21 February 2021 7,726 9 View

In methylene blue test Why is UV absorbance reading very low as soon adsorbent is added and then increasing before reaching equilibrium?

Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...

19 February 2021 472 5 View

What type of pressure sensor are usually used in microfluidics?

My team is designing a dual channel microfluidic chip that runs air and water. I think we've decided on the NE1000 syringe pump for it's versatility and feedback control. We will be ordering the...

18 February 2021 9,928 2 View

How to realize refractive index values of 1 to 1.1?

Hello researchers! While studying the literature on refractive index sensors, most of the articles simulate their devices with a refractive index ranging from 1 to 1.1, with small steps in...

17 February 2021 1,578 4 View