Hi all,
I am working with extracts of secondary metabolites of orchid species (roxburghii).
The chromatogram has many peaks that correspond to flavonoids glycosides, glycosides of hydroxycinnamic acids, carotenoids, and chlorophylls. But among them, there are several peaks with unknown spectra. Unfortunately, there are no similar spectra in our library.
Have anyone some suggestions about what kind of compound could be that?
Yes, I am sure that this peak is pure. The background signal was subtracted and the blank run doesn't have any peaks. The signals from all five wavelengths (DAD detector) show the same peak (time retention and good symmetry). Moreover, there are several peaks with similar spectra. I am thinking that this is anthocyanins or anthocyanidins
I saw similar spectra, e.g. in red cabbage and in the skin of a red radish. I think they are anthocyanins but additionally substituted with the rest of some hydroxycinnamic acid, e.g. ferulic or coumaric acid.
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