I want to carry out toxicity testing with Ceriodaphnia dubia. I've previously done this in glass beakers, but I have very limited volumes of toxicant solution, so I was hoping I could do this in 12- or 24-well microplates.
Microwell plates used for tissue culture are often surface-treated to improve cell adhesion. The type we have available in the lab states, "Tissue culture treated by vacuum gas plasma", which I'm told puts a charge on the plate surface. Does anyone know whether these plates could be used, or should I avoid them and buy in untreated plates?
Also, can anyone advise on what volume of test solution would work best, or suggest any other hints or tips for small-volume testing?
Hello Anne,
I've always used the 6 well plates in general for different species, I think they are rather versatile. You can fill them up to 10 mL, but you need a reversed microscope to read your samples.
Hope it helps,
All the best,
Andrea
https://commons.wikimedia.org/wiki/File:6_wells_cell_culture_plate-03.jpg
Thanks for the suggestion, Andrea. Have you tried plates that are surface-treated for tissue culture, or only the non-treated variety?
Thanks, Innocent. I know it went into the wrong category, just couldn't figure out how to fix it! I think I've fixed it now. Regards, Anne
Multiwell plates 6 x 4 wells are available for the test from www.microbiotests.be. I have found out that different plastic manufacturers have different procedures and materials and it is a big risk to use test plates that have not been tested for the needed purpose. Some multiwell plates express high toxicity due to manufacturing process. Regards, Juha
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