How can I solve dirtiness in my western blot ?

I am looking thrombospondin-1 protein in mouse cerebellum tissue. I can get band, but always there are dirtiness on the membrane. I tried different concentration of thrombospondin and also I used...

08 July 2020 3,321 3 View

No title

These common ID Models are ADDIE, Dick and Carey, Kemp Design, so on. (ID: Instructional Design) I would appreciate if you could share your opinion with me. Thank you! Hulya

04 June 2020 3,420 7 View

Does anyone use Agilent fragment analyser instrument (5200, 5300 or 5400 model) for STR profiling and/or cell line authentication?

cell line authentication, STR profiling, fragment analyser

22 December 2019 2,772 2 View

Is it possible to omit "neutral" option from a 5-point Likert scale?

I would appreciate if you share your opinion. Thank you!

17 September 2019 7,196 6 View

Have you ever used online coding tools? If so, could you suggest good coding resources?

Coding tools for analysing: - interview transcripts - online discussion postings - reflection reports Thank you!

09 December 2018 6,105 4 View

What strategies do you suggest adult learners for learning new words in second language?

Looking for any effective ways of vocabulary learning for foreign/second language learners... Appreciate any advice; thank you! Hulya

26 February 2018 9,862 23 View

Are there any commonly applied criteria for reviewing manuscripts for peer-review journals, barring the proposed reviewer guidelines?

Dear All, I would appreciate any comment or suggestion on this issue. Hulya

24 December 2017 9,807 8 View

What needs to be considered when wording or phrasing the emergent themes identified in the qualitative data*?

In terms of word choice, length of a phrase, structures, etc. *Data collected from the interviews. Thanks in advance.

03 May 2017 4,124 5 View

Cloning and expression vector for Bacillus subtilis....

Hi, All... I am looking for a cloning and expression vector for Bacillus subtilis. Of course I have some choices.... But let me know your experiences.. Do you have any suggestion for both...

04 September 2011 8,495 2 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View