I incubated B-CLL primary cells with EVs to study their effect on viability. After 48 hours, I measured viability with flow cytometry, I labeled my cells with Annexin V and 7AAD.
The samples incubated with EVs had a huge percentage of annexin V signal in contrast to the control, even though the EVs were supposed to be washed throughout the process of staining, I washed at least 3 times. I'm aware that some EVs bind to cells and a certain percentage couldn't be washed away, but it's too much in my flow cytometry graph.
I attached a file with the results for visualization.
Your EVs are too small to be read by Flow Cytometry unless they are coupled to something larger like a bead. Is there any reason these cells would have antibodies specific for EVs such as CD63 or CD 81? If not, then probably the EVs had a negative effect and killed off most of the cells
Dear Farah Aqel ,
Your EV's definitely have significant effect on B-CLL cells. Although, the percentage of FITC+ cells jumping from 23 to 59 % maybe explainable by correlating with the severity of B-CLL.
Please feel free to knock me for further discussion , as i'm also working on Leukemia.
Best regards,
Babu M.
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