Hi,What is the names of all organic laser dyes?

Hi,What is the names of all organic laser dyes?

16 April 2020 3,543 11 View

Method for induction stone formation in kidney of rats?

accurate method for stone formation in short time

17 September 2018 5,980 3 View

Is there soil erodibility (K) factor values specified for FAO soil classification?

I need soil erodibility (K) factor values assigned to soil type according FAO soil classification for the evaluation of soil loss using RUSLE. Thank You.

17 March 2016 1,517 6 View

How can I decide the amount of cDNA added in PCR reaction if your gene of interest is in low abundance?

I am having trouble in PCR to amplify a low abundant gene. cDNA is made from total RNA. I was always adding 2 ng/ul - 4 ng/ul cDNA in the PCR reaction. Positive control like GAPDH always shows up...

30 August 2015 3,472 6 View

How can I do overlap PCR?

I need to clone a 4kb gene. I have problem in cloning the whole gene in a single PCR reaction. So I decided to amplify two fragments then overlap the two fragments in a another round of PCR. Both...

16 April 2015 4,420 3 View

Why has PCR amplifying 4kb DNA using cDNA as a template failed?

I used the SMARTer RACE kit from clontech to get the 3' and 5 ends of my target gene. Then I tried to PCR amplify the whole gene. I tried both the platinum Taq High fidelity DNA polymerase and...

25 January 2015 5,780 13 View

Problem with misinterpretation of nanoparticle protein interaction effect on protein structure

Protein and nanoparticle interaction causes perturbation in protein structure. Many reports say that the nanoparticles, - protect the protein from denaturants like urea and Guanidine-HCl. - can...

12 June 2014 4,141 1 View

Nanoparticle concentration or dose measurement problem

During nanoparticle (like gold and silver) synthesis, we have gone through processes such as centrifugation and dialysis to remove unbound surfactant, but we do not know exact the concentration...

16 March 2014 5,026 4 View

How to prepare protein weight marker in the lab?

Protein markers are used for analysis of approximate size and weight of protein.

07 October 2013 665 9 View

Hi, could anyone suggest me which software or web server to use for epitope prediction?

Hi, could anyone suggest me which software or web server to use for epitope prediction? I have two samples, both are showing same consensus binding site from ELISA and Western Blot. Thus, I am...

08 September 2012 2,572 9 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

How to write a scientific report?

We are analysing scientific reports at University in great detail, so I wanted to find a scientific report about how to write a scientific report. Including detail such as what information to put...

02 March 2021 7,602 4 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

No title

01 March 2021 3,905 5 View

How to Balance Bench work with Reading and Writing?

I am a second-year grad student. I am finding it difficult finding time to read literature and write (things like qualifying exam, thesis, grants). I find myself so busy with bench work that I...

01 March 2021 3,325 4 View

Which software is better to write systematic review paper?

We have seen some authors use some software to write review paper. As obvious, the study of all published papers concerning our studying field is not possible and these software can help us to...

27 February 2021 6,296 1 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View

What are the important tips for writing review articles?

Important tips for writing a good review articles

24 February 2021 8,544 6 View