What time of day is best to take soil respiration samples?

More Bryan Driscoll's questions See All

How do you prevent excessive foaming of organic-matter-rich soils during H2O2 digestion?

My samples are very high in organic matter, and even small amounts of H2O2 cause the samples to foam up 4-5 times the beaker volume, spilling onto the lab bench. Is there anything that can be done...

17 January 2021 8,784 3 View

Does it matter if antibodies are monoclonal or polyclonal for Quantitative Western Blots?

Hello helpful people, I am planning a quantitative western blot experiment and have the choice between a polyclonal and monoclonal antibody. I have never done western blots personally. Does...

11 November 2020 780 4 View

How to determine fluorescence quantum yield for photoswitchable/photochromic compounds?

How to determine FQY for photoswitchable compounds? I found most related publications didnt mention, or just said that they used "Absolute quantum yield spectrometer." I think such technique...

31 October 2020 8,002 5 View

Will intron-mediated enhancement of gene expression affect downstream of a fusion protein?

Hello everyone! I'm currently trying to increase the expression of my fusion protein in a C. elegans animal model. After reading papers, I found two possible methods to try: (1) codon-optimization...

29 September 2020 490 2 View

Should we include 2 clinically correlated variables (Correlation constant = 0.8) in the same Multi-linear regression model?

I am currently studying the effect of atrophy of a muscle on the clinical outcome of joint injury. There is actually another muscle that was previously well established to have an effect on...

06 September 2020 9,986 7 View

What could be an explanation for making plants in their growth development appear fake growth rings?

As plants grow in their development, they undergo many changes either due to climate change or other environmental issues that affect plants but I want to know how false growth rings form and why...

16 December 2019 1,615 5 View

Primers for amplifying Lactadherin?

Has anyone had previous success in amplifying Lactadherin from the addgene construct below? https://www.addgene.org/46830/ I'm looking at the sequence and it seems that the starting sequence of...

11 October 2019 1,158 3 View

Is there a straight forward way to modify the amine of an amino acid?

It's been a while since I've thought a ton about synthetic organic chemistry, so please for give my naivete. For example, say I wanted to add an R group to the amine group on glycine. I believe...

24 August 2019 10,011 3 View

Type of analysis for three IVs combine and correlate to DV?

I am attempting to figure out the correct type of analysis I would use for the following nominal IV/DV situation. I predict that a person who commits a heinous crime has experienced the...

05 May 2019 3,011 3 View

Why would media stain cells?

I'm working with mesenchymal stem cells, and made an observation that I'm scratching my head to explain. After spinning down cells and washing in PBS the cell pellet for WT cells is white, but...

06 January 2019 4,035 3 View

How can the use of Jolly Phonics improves the quality of reading, speaking and writing in rural disadvantaged communities in Papua New Guinea?

Jolly Phonics reading program is an effective way to teach early reading to young learners. How can lectures at the university prepare early childhood student teachers to teach Jolly Phonics to...

03 March 2021 3,731 3 View

How to quantify carbon deposition on CuZnAl catalyst?

Dear All, I want to quantify carbon deposition on the surface of the CuZnAl catalyst. I have tried thermogravimetric analysis, but I've got weight gain as the active Cu- get oxidised. Does anyone...

01 March 2021 1,907 3 View

Why Trivalent chromium start precipitating above PH 5 ? Moreover discuss difference between adsorption of chromium and precipitation of chromium ?

I am a student of MS Environmental Engineering and started my research phase. I am working on metal removal ( chromium iii ) by using activated carbon prepared from the agricultural feedstock. So...

01 March 2021 5,475 2 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

CGA 300 Regulator for CO2 cylinder?

Has anyone ever tried to deliver CO2 with a CGA 300 regulator? I know that a CGA 320 regulator is standard for CO2. The issue is that I currently do not have any free CGA 320 regulators. I also...

25 February 2021 3,292 3 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

What are the methods used for CNT..?

Respected Professor's i hope you are doing well and happy. i need some information about carbon Nanotubes. before blending the CNT with the matrix the CNT has been introducing in some treatments....

24 February 2021 5,383 5 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

Have anyone checked before Cyclic voltammograms of intial ITO substrate and E-teck?

As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...

23 February 2021 3,175 3 View

Baljit Singh

I think it should be taken during morning hours and mid day and then average of these two values can be quoted.

Chimi Djomo Cédric

Seeing that you will use EGM-5... To mesure, I suggest you to take at different and make your

conclusion

Ashok Kumar Singh

It would be better to take one sample in the morning , another one at mid day and last one in the evening and take average value of all three samples, will give you better result.