i need the exact protocol for preparing the rhodamine B dye
to concentration of 0.1% and the quanitity needed for mixing 0.1% of rhodamine B dye to root canal sealer to examine it under conofocal laser scanning microscope (NIkon,Japan)
also is the dye altering the flow and setting time of the root canal sealer ?
this is all information i have about Rhodamine B dye
Rhodamine B from sigma aldrich
≥95% (HPLC)
Synonym: Basic Violet 10, Brilliant Pink B, Rhodamine O, Tetraethylrhodamine
Formula: C28H31ClN2O3
mp 210-211 (dec.)(lit.) solubility H2O: 1 mg/mL absorption extinction/542-554 nm, methanol 104500-115800
and i will use Sure Seal bioceramic root canal sealer from Sure Dent, Korea
and AH Plus from Dentsply
Dear Dina,
It seems that you are not familiar with the posting of a R.G. Question! Why?
Rhodamine B : (It is a trivial name) !
At first you must give the counter anion of the rhodamine B dye in your question.
P.e. Rhodamine B Chloride (see added file) or isothiocyanate (see link) :
How to cite this URL: Ravi S V, Nageswar R, Swapna H, Sreekant P, Ranjith M, Mahidhar S. Epiphany sealer penetration into dentinal tubules: Confocal laser scanning microscopic study. J Conserv Dent [serial online] 2014 [cited 2018 Apr 12];17:179-82. Available from: http://www.jcd.org.in/text.asp?2014/17/2/179/128056
and define your root canal sealer (see link):
http://endomatters.dental/what-to-look-for-in-a-root-canal-sealer/
we, answerers, need these infos for help you in your research.
Thank you in advance.
JRG
i have edited my question to be more clear
sorry if i'm familiar with the posting of R.G question it's my first time here
thanks all and i hope you can help me
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