How to extract nuclei from OCT frozen tissue?

I am trying to extract intact nuclei from OCT-frozen tumor tissue samples. Does anybody have a protocol for doing so? I need as many intact nuclei as possible for performing ATAC-seq on them.

13 September 2020 8,608 1 View

Determination of dc electrical resistivity from FMR lineshape?

Suppose I have FMR line shapes (dP/dH) at different temperatures for a particular frequency, say 10 GHz. If the line shape contains both absorptive and dispersive components, we can fit the line...

27 May 2020 4,140 1 View

How to dissolve a 21-mer synthetic peptide (acidic)?

I'm facing a problem in dissolving two synthetic peptides (21 amino acids long). The peptides have iso-electric points at pH 4.01 and good predicted solubility in water. However, the synthesized...

16 February 2020 6,617 3 View

How do you write personal experiences in a textbook chapter in first person when there are two authors?

I am currently working on a textbook chapter with another author. The editors have asked us to use personal examples and experiences with the information and write in first person, but I am...

12 October 2019 9,740 7 View

What is communication management in project management?

Good Morning. I have an assignment based on the project management and they told us to write a report based on the topic given above on a particular social media company. I would be very...

24 July 2018 5,994 5 View

I need help identifying the specific error variable on CFD post?

I am simulating a 3D cavitation model with 2 Way FSI coupling in ANSYS Fluent 18.1. The simulation is succesful but as I open CFD post for post processing, I get this error. Variable 'Pressure'...

10 December 2017 7,076 3 View

How to statistically calculate the number of ML/BI trees required for a phylogenetic analysis?

I am currently doing a phylogenetic analysis for a species of virus having many subtypes (Human papillomavirus) for around 100 whole genome specimen and 50 partial sequences belonging to ~6...

01 December 2017 8,176 2 View

How does digestion affect the efficacy of shearing?

Hello, I am preparing RADseq libraries. I am shearing digested DNA (mean size 4096bp) using a Covaris ME220. The shearing is not working as fast as it should (compared to undigested DNA). Does...

15 November 2016 9,279 4 View

How is a confidence interval calculated for an Area Under the Curve (ROC)?

I don't see a CI in the SPSS output, even though I selected that option, and want to test the significance of the difference between two AUC's.

05 July 2016 1,348 8 View

What type of Cl- salt should I use when I have decreased NaCl in order to make a Na+ low solution?

I want to decrease the concentration of Na+ in my extracellular solution. I have therefore decreased the amount of NaCl in my solution. What Chloride salt shoudl I use to maintain the same amount...

03 January 2016 6,988 5 View

How to add a journal?

I would like to know how to register the journal Flora Montiberica (ISSN 1138-5952 - eISSN 1988-799X) in Research Gate.

02 March 2021 8,156 2 View

Blast analysis of RC,UHPC slabs using material model *MAT_72R3 in LS-DYNA?

20 February 2021 5,984 3 View

Good day sir/ma. Please, where can I have access to equipment for conducting XRF, XRD and SEM analysis?

I'm using blast furnace slag for stabilizing subgrade soil.

17 February 2021 7,333 3 View

Is nr blast important?

Hello, I have designed a specific primer for my Gene, when I blast this primer, it just finds the mRNA of the gene, but in nr blast, I have found some CDSs that are not relevant to my Gene. Do...

05 February 2021 5,842 3 View

Abaqus - plate is going up under gravity+blast loads?

Hi, I have modeled concrete plate with reinforcement supported on 2 concrete block. between concrete block and the plate I have added regular contact. I have created 2 steps: 1) only gravity load...

30 January 2021 2,772 1 View

Is there any alternative to BUSCO for searching orthologues within genomes available?

I have a need to search for single-copy conservative orthologs within several plant genomes and used BUSCO tool for that. However, I found that the results produced by different versions on the...

29 January 2021 7,764 6 View

How to check "no blast hit" sequences of NGS data output?

My environmental sample was analyzed for fungi by both DGGE (18S) and NGS (ITS2), resulted in some different names (in the top 5 of strong bands in DGGE, or most abundant sequences in NGS) between...

21 January 2021 5,310 3 View

Best way to optimize species-specific primers? Can I use a temp gradient to test a Ta where I will get target (and no non-target) amplification?

Hello, I am testing different species-specific primer sets. I have two targets (two species, but same gene target). I am trying to select the best primers -- one that will amplify the target...

18 January 2021 1,981 6 View

Can BLAST/ search by using known Domain in Transcriptome/Genome data for a gene family?

Dear Researchers, I have Transcriptome/Genome data and want to list all the member of a KCS/CER gene family whose Domain AP2/ERF, FAE1_CUT1, according to the Arabidopsis database, so is there...

15 January 2021 2,293 3 View

How can I build a local KEGG db?

I want the info (gene name, metabolic pathway, and organism of origin) of some key identified metatranscriptome genes obtained from MG Rast (I have the sequences). The KEGG ftp site seems to...

12 January 2021 9,985 1 View