I am carrying out fatty acid analysis on 3 different pasture mixes. I have a 6 species mix, a 2 species mix and I have divided a portion of the 6 species mix into its individual constituents. In total I have perennial ryegrass, timothy, white clover, red clover, chicory, plantain, 6 species mix and 2 species mix, meaning I have 8 samples in total. I want to look at the differences between the fatty acid profile of the species and then how this changes over time. Initially I thought I would be able to perform ANOVA to analyse the differences between the fatty acid contents of the species, however, the sample prep involved pooling pasture samples from 3 different fields which means the triplicate samples I prepared are from the pooled mix rather than 3 separate samples, therefore I don't think I can perform this. If anyone has any suggestions it would be greatly appreciated.
Kindly check https://onlinelibrary.wiley.com/doi/10.1111/fwb.13315
Also check https://besjournals.onlinelibrary.wiley.com/doi/10.1111/2041-210X.12869
See this article
Fatty acids differentiate consumers despite variation within prey fatty acid profiles
Hi
The analysis and calculations protocols are well explanined in the following paper:
https://doi.org/10.1080/10942912.2016.1190744
I hope this helps!
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