I run enzyme immuno-assay (EIA) to quantify hormone concentrations in samples and have used a nitrogen gas drying method in the past to dry down the extracted hormones from 2ml of 100% HPLC methanol. I no longer have access to a nitrogen tank and drying manifold in my new lab, but we do have a Thermo Fisher SPD-1030 SpeedVac. I have looked through countless papers and they all say "dried down using SpeedVac" but give no details on the temperature, vacuum pressure, or time settings. I have been trying to dry my samples using a temperature setting of 35C and 5.1 Torr, but even after almost 2 hours of drying, the sample volume has hardly changed. I do not want to go higher than 35C, as warmer temps may affect the hormones present. Any suggestions would be appreciated.

Let me also say that I have looked through the questions here on ResearchGate and some have been helpful, but still looking for more concrete answers. Some answered questions have said no heat is needed, but 35C will speed up the process. Others have said to go hotter. However, many did not mention adjusting the vacuum pressure. The SpeedVac has a minimum of 5.1 Torr and minimum 35C unless turned off completely.