I am trying to analyse the chemical profile of defensive droplets from the lepidopteran larvae (T Trypanophora semihyalina ). when i collected and tried to dilute in the hexane and acetone the secretion is getting gelly and it is not dissolving. So looking for a suitable solvent.
DearHanamant Gadad
I've used for other insects ethanol
Regards, Pushkin Sergey Viktorovich
Please read the following article link to know defense mechanism in lepidopteran.
Article Lepidopteran defence droplets - A composite physical and che...
Lepidopteran droplets are chemically peptide, protein and glucose. as Neutral peptides usually dissolve in organic solvents. You can dilute the defense droplets: First, try to add a small amount of acetonitrile, methanol, or isopropanol. For very hydrophobic peptides, try to dissolve the peptide in a small amount and then dilute the solution with water to the desired concentration.
If the peptide is basic and the total number of charges of the peptide at pH 7 is between 10–25% of the total number of residues, add a small amount of 25% acetic acid to dissolve the peptideand dilute it with water to the desired concentration.
furthermore you can also get help by clicking on the following link.
https://www.lifetein.com/handling_and_storage_of_synthetic_peptides.html
The glandular secretions of Lepidoptera contain several aromatics, biogenic amines (histamine), glycerol and trehalose, so to dissolve them you should pay attention to the content in these fluids. Dilution of the liquid needs to be tried using several solvents to find the best or a particular solvent that has been used.
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