I am a PhD student I am studying varying cryopreservation methods for sperm.

As part of this project I have used Cryo-scanning electron microscopy to investigate varying ice-crystal formation between treatment groups and to compare ice-crystal structure to the post-thaw survival cryopreserved sperm.

I have been looking into software for analysis of Cryo-SEM images to produce some quantifiable data that can demonstrate differences in the images. I have attached two images of different freezing techniques which subjectively look quite different. With these images some of the “differences” we would like to quantify include.

• I have labeled these images for a better frame of reference.

Any suggestion you have would be greatly appreciated. We have considered using ImageJ (Fiji) but was wondering if there is and kind of more advance image analysis that anyone is aware of?

Thank you very much for your time and consideration