I want to genotype 3 specific SNPs from a single gene in about 150 human patients. I want the cheapest method, I'm a university student and I'm going to pay it by my own.
Thanks for your time and help.
In that case, RFLP-PCR is your best bet. The cheapest yet one of the most precise methods out there. You can't go wrong with it.
Dear Romina,
I am not aware about the costs in Peru, but as other persons mentioned RFLP-PCR would be the choice. But this is a general answer, that should depend on which regions you are checking, what are the mutations (substitutions, indels, repetitions), how big is the gene, if you can adjust multiplex, how many repetitions for each patient you will use (doubles, triples), how good are the enzymes, do they produce same results etc etc etc.
So, in some cases sequencing might be cheaper with exact results. It will be necessary for you just to perform PCR of the region.
If you will provide more details I think you will get more support and advices.
Good luck!
Definitely, PCR-RFLP or ARMS PCR would be the best... But I would like to mention if all the three SNPs are in vicinity enough to amplify in a single PCR, then PCR-Sequencing is also a cheap option. This will also give you the added information about any other polymorphisms located in the amplicon.
- Badges
- Science topic
- Similar topics
- Correction