I have 3 extract of the same plant.
Aqueous extract - dissolves in saline
Methanol & Ethyl acetate extract - Won't dissolve in saline, needs DMSO.
Now if i decide to do a experiment using these 3 extracts, what should i use as control? Saline or DMSO diluted in Saline?
Should i dissolve all of those 3 extract in DMSO and then use DMSO as control OR dissolve the aqueous extract in saline and the 2 organic extract in DMSO and then have 2 control groups, one with saline and another with DMSO?
Thanks in advance, i did ask a relevant question before but i am still a bit confused.
In my opinion, two seperate sham groups; one with saline and one with DMSO. If your statistics software does not "create problems" to your predicted results, let your "control" groups be less in number than the other groups for animal welfare reasons (3 R's, Reduction)
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