What should I do after RNase A treatment when I purify dsRNA from total RNA?

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Andrew Hammond

I would treat the samples with proteinase K to digest the RNase A and then perform a phenol-chloroform extraction of the dsRNA from the mixture.

Daniel Dehany Scott

The other option is to use a heat-inactivatable ssRNA-specific RNAse, for example RNAse I?

Dung Viet Nguyen

You can cleanup your RNA by using RNeasy mini kit . Cleanup protocol is on page 54 of RNeasy® Mini Handbook http://www.qiagen.com/resources/download.aspx?id=14e7cf6e-521a-4cf7-8cbc-bf9f6fa33e24&lang=en