Daniel Dehany Scott

6 Questions 61 Answers 0 Followers

Questions related from Daniel Dehany Scott

Questions regarding optimising the Golden Gate cloning method?

Hi all, I'm currently in the process of constructing a number of plasmids from a series of interchangeable PCR-amplified constructs and, having done some reading, I am keen to try and apply the...

11 December 2016 3,024 5 View

One-plasmid system for dual-gRNA, D10A-nickase CRISPR?

Hi all, I'm currently designing constructs for CRISPR- and HR-mediated epitope tagging of ORFs in their genomic context, and could do with a little guidance. From what I've read, it sounds like...

03 December 2016 283 2 View

What is the best way to define promoter targets of two (or more) different transcription factors?

Hi there, I'm performing some (very basic) bioinformatic analysis of our gene of interest, part of which is looking at the different transcription factors that may regulate it using the ENCODE...

03 October 2015 4,201 10 View

What is the p53 deletion in HCT116 p53-/- cells?

In our current project we are using the HCT116 p53-/- cells originally created by homologous recombination by Bunz et al. (1998). However, despite my best efforts I've been unable to find out the...

08 August 2014 6,395 9 View

What are the advantages of tBHP over H2O2 for inducing ROS in cell lines?

My lab is currently conducting assays on the sensitivity to ROS of a couple of cell lines, and we are looking at the different agents used to induce ROS. I've heard that tBHP (tert-Butyl...

15 May 2014 8,685 4 View

Where can I find data on the total number of academics and/or research students per country?

I have developed a database of members of our organisation; these members are drawn from about 150 countries around the world and are mostly from academia or para-academic professions (e.g....

13 September 2013 6,670 10 View