Daniel Dehany Scott

5 Questions 52 Answers 0 Followers

Questions related from Daniel Dehany Scott

Questions regarding optimising the Golden Gate cloning method?

Hi all, I'm currently in the process of constructing a number of plasmids from a series of interchangeable PCR-amplified constructs and, having done some reading, I am keen to try and apply the...

11 December 2016 3,016 5 View

One-plasmid system for dual-gRNA, D10A-nickase CRISPR?

Hi all, I'm currently designing constructs for CRISPR- and HR-mediated epitope tagging of ORFs in their genomic context, and could do with a little guidance. From what I've read, it sounds like...

03 December 2016 275 2 View

What is the best way to define promoter targets of two (or more) different transcription factors?

Hi there, I'm performing some (very basic) bioinformatic analysis of our gene of interest, part of which is looking at the different transcription factors that may regulate it using the ENCODE...

03 October 2015 4,193 10 View

What are the advantages of tBHP over H2O2 for inducing ROS in cell lines?

My lab is currently conducting assays on the sensitivity to ROS of a couple of cell lines, and we are looking at the different agents used to induce ROS. I've heard that tBHP (tert-Butyl...

15 May 2014 8,676 4 View

Where can I find data on the total number of academics and/or research students per country?

I have developed a database of members of our organisation; these members are drawn from about 150 countries around the world and are mostly from academia or para-academic professions (e.g....

13 September 2013 6,663 10 View