What should be the storage condition for PVDF Powder?

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I am starting tp learn molecular docking but am stuck on making a .map file by autogrid, how do I get past this error?

This is what it shows on my computer. Please help

05 June 2024 6,187 7 View

Mitochondria Isolation from human skin fibroblasts?

Dear All We are trying to isolate mitochondria from human skin fibroblasts by applying various protocols involving douncing. We tried to use nearly 50 million cells, used different douncers, and...

17 March 2024 4,804 1 View

How to calculate Treatment cost ? how to scale up the treatment technology for Rhodamine B dye degradation application ?

How to calculate Treatment cost and how to scale up the treatment technology for dye degradation application .

01 January 2024 5,511 0 View

What is the difference between the Lienard based oscillators and Consensus based osillators?

The virtual oscillator based grid forming inverter topologies are broadly divided into two categories. First one is Lienard oscillators (Van der Pol, Dead-zone). The second one is Consensus based...

12 December 2023 9,817 0 View

Iam working on encapsulation of Multiwalled CNTs.. Since the encapsulation complex is in dmso solvent, How to dry the material?

Which solvent is used to disperse cnt material for the drop cast in cv?

30 November 2023 9,781 2 View

How can we determine the formation of N-oxide , sulfoxide or sulfone using spectroscopy?

I have a molecule having N and S atoms, applying oxidation methods gives me 3 spots on TLC, how can I I determine the pr sence of N-oxide, sulfoxide or sulfone in it?

09 November 2023 8,572 1 View

Mitochondria Isolation from human dermal fibroblasts?

Dear Community We have been unable to extract sufficient mitochondria from ~10 million cells with a glass-glass douncer. We believe we are missing something in the protocol. Would be glad if...

09 July 2023 7,417 3 View

Small sample control and structural safety (Characteristic Strength of concrete)?

Is the following report available some where? Can some one provide a copy of the same. Öfverbeck, P., Small sample control and structural safety. Rep. TVBK – 3009, Dept. of Struct. Engrg., Lund...

20 March 2023 3,524 0 View

What are the challenge faced by the gig workers ?

Gig workers is type of worker community who work on contract basis and Employer is not guaranteed on job security.

14 March 2023 1,890 8 View

What are author rights related to pre-print of a paper/chapter ?

I want to know, Is it author's universal right to publish an pre-print of a paper in open-access repositories or it depends on various publishers policies. I am asking in the context of...

03 March 2023 2,547 3 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Can i use the protease inhibitors during cell membrane vesicle preparation ？?

I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...

30 July 2024 7,077 1 View

What is the reason I get 400nm nanoparticles using an extruder with 200 nm membrane for cell membrane coating?

I am trying to coat my micelles and PLGA based nanoparticles with cell membrane using an Avanti mini extruder according to literature . I use 200nm membrane but my particles end up to be 400nm And...

30 July 2024 3,294 3 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,624 1 View

After regeneration studies of the PVDF membrane, will the permeate flux and TDS removal efficiency increase or decrease?

Regeneration Studies for the PVDF membrane and PVDF/CuO nanocomposite membrane were carried out by chemical cleaning.

07 July 2024 7,033 0 View

What does it mean when NucBlue disappears completely during live cell imaging?

Using confocal imaging, I'm investigating the effect of a particular compound on mitochondrial membrane potential in live cells. I've stained the cells with varying concentrations of the compound...

29 June 2024 7,546 4 View

How to prepare Gold nanoparticle sample for XRD?

We have been lyophilizing several nanoparticles like iron oxide, cerium oxide to get powder sample. However, for gold nanoparticles and any other NPs with gold, they turn sticky after lyophilizing...

26 June 2024 9,171 3 View

Please, whoever has this part of the book, send it to my email, because I need it and cannot buy it?

Strontium: Source, Occurrence, Properties, and Detection Chapter First Online: 05 June 2019

25 June 2024 2,198 0 View

How to fix cells with glutaraldehyde for immunofluorescent staining?

Hello, I am trying to fix primary cells with glutaraldehyde. I am trying to stain cell membrane proteins and I have not been successful with PFA and methanol fixation. Does anyone have a protocol...

17 June 2024 3,434 5 View

Luciferase assay lysis?

Hi! I'm doing a renilla luciferase assay with coelenterazine and HEK293T cells transfected with AP-1 renilla luciferase. I'm wondering if the cells need to be lysed prior to adding coelenterazine...

13 June 2024 9,936 5 View

Jasim Ahmed

Sahil: I check in the website of Sigma-Aldrich and its moisture content is less than 2%. Therefore, you have to be careful as it is highly hygroscopic so you need an airtight container like baby food or milk powder. It is better seal it properly and keep in referigerator or room temp if it is less than 25C.

Sahil Thareja

Thankks a lot Jasim Ahmed Sir